KEY RESOURCE TABLE
| REAGENT or RESOURCE | SOURCE | IDENTIFIER |
|---|---|---|
| Antibodies | ||
| Rabbit anti-TDP-43 (polyclonal) | Proteintech | Cat# 10782–2-AP, RRID: AB_615042 |
| Rabbit anti-TDP-43 (polyclonal) | Proteintech | Cat# 12892–1-AP, RRID: AB_2200505 |
| Mouse anti-GFP (monoclonal, JL-8) | Clontech | Cat# 632381, RRID: AB_2313808 |
| Mouse anti-G3BP1 (monoclonal) | Abcam | Cat# ab56574, RRID: AB_941699 |
| Goat anti-TIA1 (polyclonal) | Santa Cruz | Cat# sc-1751, RRID: AB_2201433 |
| Mouse anti-HA (monoclonal, HA.11) | Covance | Cat# mms-101P, RRID: AB_2314672 |
| Rat anti-tubulin (monoclonal) | Abcam | Cat# ab6160, RRID: AB_305328 |
| Mouse anti-GAPDH (monoclonal) | Abcam | Cat# ab8245, RRID: AB_2107448 |
| Rat anti-phospho-TDP-43 | Biolegend | Cat# 829901, RRID: AB_2564934 |
| Goat anti-RanGap1 | Santa Cruz | Cat# sc-1862, RRID: AB_2176981 |
| Mouse anti-Ran | BD Bioscience | Cat# bd-610340, RRID: AB_397730 |
| Rabbit anti-Nup107 | Abcam | Cat# Ab73290, RRID: AB_1269604 |
| Goat anti-FUS | Bethyl Laboratories | Cat# A303–839A, RRID: AB_2620190 |
| Mouse anti-hnRNPA1 | Santa Cruz | Cat# sc-374053, RRID: AB_10947257 |
| Rabbit anti-amyloid A11 | ThermoFisher | Cat# AHB0052, RRID: AB_1501357 |
| Rabbit anti-peIF2a | Cell Signaling | Cat# 3398, RRID: AB_2096481 |
| Guinea pig anti-p62 | Progen | Cat# GP62-C, RRID: AB_2687531 |
| Mouse anti-EDC4 | Santa Cruz | Cat# sc-376382, RRID: AB_10988077 |
| Mouse anti-ubiquitin | Millipore | Cat# MAB1510, RRID: AB_2180556 |
| Mouse anti-Importin alpha 2 | Novus Biologicals | Cat# MAB6207, RRID: AB_10730707 |
| Chicken MAP2 | Novus Biologicals | Cat# NB300–213 RRID:AB_2138178 |
| Bacterial and Virus Strains | ||
| E. coli DH 5-alpha competent cells | New England Biolabs | Cat# 2981I |
| E. coli BL21 competent cells | EMD millipore | Cat# 69450–3 |
| Biological Samples | ||
| Chemicals, Peptides, and Recombinant Proteins | ||
| Protamine sulfate | Sigma-Aldrich | Cat# P4020 |
| TransIT-X2 transfection reagent | Mirus | Cat# MIR 6000 |
| Sodium arsenite | Sigma-Aldrich | Cat# 1062771000 |
| PD 0332991 (Palbociclib) | Apexbio | Cat# PD-0332991 |
| Carbonic Anhydrase from bovine erythrocytes | Sigma-Aldrich | Cat# C2273–1VL |
| Albumin, bovine serum | Sigma-Aldrich | Cat# A8531 |
| B-Amylase from sweet potato | Sigma-Aldrich | Cat# A8781 |
| TEV protease | ThermoFisher | Cat# 12575015 |
| Poly-D-lysine | Sigma-Aldrich | Cat# P0899 |
| Laminin | Corning | Cat# 354232 |
| 20% paraformaldehyde solution, EM grade | Fisher Scientific | Cat# 15713S |
| Neomycin | Thermo Fisher | Cat# 10131035 |
| Blasticidin | Thermo Fisher | Cat# A1113903 |
| Puromycin Dihydrochloride | Thermo Fisher | Cat# A1113803 |
| Doxycycline | Sigma-Aldrich | Cat# D9891–5G |
| Uranyl acetate | Ladd R.I., Williston, VT | Cat# 23620 |
| Antibiotic-Antimycotic | Thermo Fisher | Cat# 15240062 |
| Fetal Bovine Serum | Omega Scientific | Cat# FB-01 |
| Critical Commercial Assays | ||
| Deposited Data | ||
| Experimental Models: Cell Lines | ||
| iPS Cells | iXCells | N/A |
| Human: SH-SY5Y | ATCC | Cat# CRL-2266 |
| Human: SH-SY5Y TDP-43AID-GFP | This paper | N/A |
| Human: U2OS | ATCC | Cat# HTB-96 |
| Human: HEK293T | ATCC | Cat# CRL-3216 |
| Experimental Models: Organisms/Strains | ||
| C57BL/6 (C57BL/6 NCrL) mice | Charles River Laboratories | Cat# C57BL/6NCrl |
| Oligonucleotides | ||
| Synthetic RNA poluCUG repeat (5X) | IDT | N/A |
| Oligo-dT(20) | Gift from Dr. J. Paul Taylor (St. Jude Children Hospital) | N/A |
| Recombinant DNA | ||
| pDUET-HA-FUSWT | This paper | N/A |
| pET His-TDP-43WT | This paper | N/A |
| pET His-SOD1A4V | This paper | N/A |
| pRK2- His-FUSWT-mCherry | This paper | N/A |
| pRK2-His-TDP-43WT-mCherry | This paper | N/A |
| pRK2-His-TDP-43G298S-mCherry | This paper | N/A |
| pRK2-His-SOD1G93R-mCherry | This paper | N/A |
| pX330-U6-Chimeric_BB-CBh-hSpCas9 | Addgene Cat# 42230 | (Cong et al., 2013) |
| Lentivirus | ||
| EF1a-Tet3G-IRES-Neo | This paper | N/A |
| Ubic-myc-TDP-43WT-mRuby2-SV40-Puro | This paper | N/A |
| TRE3G-TDP-43ΔNLS(K82A,R83A,K84A)1−414-Clover-EF1acore-BSD | This paper | N/A |
| Ubic-UBAP2L-mRuby2-SV40-Puro | This paper | N/A |
| Ubic-TDP-43ΔNLS(K82A,R83A,K84A)1−414-Clover-SV40-Puro | This paper | N/A |
| pCDH-CMVtet-EYFP-TDP-43 | This paper | N/A |
| Software and Algorithms | ||
| FIJI (ImageJ) | NIH | Fiji, RRID: SCR_002285 https://fiji.sc/#download |
| Benchlin webtool | Benchling | https://benchling.com/signup |
| Graphpad prism | Graphpad | Graphpad Prism RRID:SCR_002798 https://www.graphpad.com/scientific-software/prism/ |
| Other | ||
| 96 well glass bottom plate | Cellvis | Cat# P96–1.5H-N |
| 8 well chamber slides (glass bottom) | Ibidi | Cat# 80827 |
| GSTrap HP columns | GE | Cat# 17–5281-01 |
| HisTrapHP colums | GE | Cat# 7–5248-02 |
| Superdex 75 10/300 | GE | Cat# 17–5174-01 |
| Sephadex G-25 in PD-10 | GE | Cat# 17–0851-01 |
| Hemacytometer | Fisher Scientific | Cat# 267110 |
| DeltaVision Core system | GE | N/A |
| Olympus FV1000 Spectral Confocal with SIM Scanner | Olympus | N/A |
| Tecnai G2 Spirit BioTWIN transmission electron microscope equipped | Tecai | N/A |
| Zeiss LSM 880 confocal microscope | Zeiss | N/A |
| CQ1 confocal quantitative image cytometer | Yokogawa | N/A |
| SH800S Sony cell sorter | Sony | N/A |
| JEOL 1200 EX II TEM | JEOL USA | N/A |
| Akta Pure | GE | N/A |
| Amaxa Nucleofactor | Lonza | N/A |
| C-B 300 mesh Cu-01813-F | Ted pella | Cat# Cu-01813-F |