Antibodies |
Rabbit anti-TDP-43 (polyclonal) |
Proteintech |
Cat# 10782–2-AP, RRID: AB_615042 |
Rabbit anti-TDP-43 (polyclonal) |
Proteintech |
Cat# 12892–1-AP, RRID: AB_2200505 |
Mouse anti-GFP (monoclonal, JL-8) |
Clontech |
Cat# 632381, RRID: AB_2313808 |
Mouse anti-G3BP1 (monoclonal) |
Abcam |
Cat# ab56574, RRID: AB_941699 |
Goat anti-TIA1 (polyclonal) |
Santa Cruz |
Cat# sc-1751, RRID: AB_2201433 |
Mouse anti-HA (monoclonal, HA.11) |
Covance |
Cat# mms-101P, RRID: AB_2314672 |
Rat anti-tubulin (monoclonal) |
Abcam |
Cat# ab6160, RRID: AB_305328 |
Mouse anti-GAPDH (monoclonal) |
Abcam |
Cat# ab8245, RRID: AB_2107448 |
Rat anti-phospho-TDP-43 |
Biolegend |
Cat# 829901, RRID: AB_2564934 |
Goat anti-RanGap1 |
Santa Cruz |
Cat# sc-1862, RRID: AB_2176981 |
Mouse anti-Ran |
BD Bioscience |
Cat# bd-610340, RRID: AB_397730 |
Rabbit anti-Nup107 |
Abcam |
Cat# Ab73290, RRID: AB_1269604 |
Goat anti-FUS |
Bethyl Laboratories |
Cat# A303–839A, RRID: AB_2620190 |
Mouse anti-hnRNPA1 |
Santa Cruz |
Cat# sc-374053, RRID: AB_10947257 |
Rabbit anti-amyloid A11 |
ThermoFisher |
Cat# AHB0052, RRID: AB_1501357 |
Rabbit anti-peIF2a |
Cell Signaling |
Cat# 3398, RRID: AB_2096481 |
Guinea pig anti-p62 |
Progen |
Cat# GP62-C, RRID: AB_2687531 |
Mouse anti-EDC4 |
Santa Cruz |
Cat# sc-376382, RRID: AB_10988077 |
Mouse anti-ubiquitin |
Millipore |
Cat# MAB1510, RRID: AB_2180556 |
Mouse anti-Importin alpha 2 |
Novus Biologicals |
Cat# MAB6207, RRID: AB_10730707 |
Chicken MAP2 |
Novus Biologicals |
Cat# NB300–213 RRID:AB_2138178 |
|
|
|
Bacterial and Virus Strains |
E. coli DH 5-alpha competent cells |
New England Biolabs |
Cat# 2981I |
E. coli BL21 competent cells |
EMD millipore |
Cat# 69450–3
|
|
|
|
|
|
|
Biological Samples |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Chemicals, Peptides, and Recombinant Proteins |
Protamine sulfate |
Sigma-Aldrich |
Cat# P4020 |
TransIT-X2 transfection reagent |
Mirus |
Cat# MIR 6000 |
Sodium arsenite |
Sigma-Aldrich |
Cat# 1062771000 |
PD 0332991 (Palbociclib) |
Apexbio |
Cat# PD-0332991 |
Carbonic Anhydrase from bovine erythrocytes |
Sigma-Aldrich |
Cat# C2273–1VL |
Albumin, bovine serum |
Sigma-Aldrich |
Cat# A8531 |
B-Amylase from sweet potato |
Sigma-Aldrich |
Cat# A8781 |
TEV protease |
ThermoFisher |
Cat# 12575015 |
Poly-D-lysine |
Sigma-Aldrich |
Cat# P0899 |
Laminin |
Corning |
Cat# 354232 |
20% paraformaldehyde solution, EM grade |
Fisher Scientific |
Cat# 15713S |
Neomycin |
Thermo Fisher |
Cat# 10131035 |
Blasticidin |
Thermo Fisher |
Cat# A1113903 |
Puromycin Dihydrochloride |
Thermo Fisher |
Cat# A1113803 |
Doxycycline |
Sigma-Aldrich |
Cat# D9891–5G |
Uranyl acetate |
Ladd R.I., Williston, VT |
Cat# 23620 |
Antibiotic-Antimycotic |
Thermo Fisher |
Cat# 15240062 |
Fetal Bovine Serum |
Omega Scientific |
Cat# FB-01 |
Critical Commercial Assays |
|
|
|
|
|
|
|
|
|
|
|
|
|
|
|
Deposited Data |
|
|
|
|
|
|
|
|
|
Experimental Models: Cell Lines |
iPS Cells |
iXCells |
N/A |
Human: SH-SY5Y |
ATCC |
Cat# CRL-2266 |
Human: SH-SY5Y TDP-43AID-GFP
|
This paper |
N/A |
Human: U2OS |
ATCC |
Cat# HTB-96 |
Human: HEK293T |
ATCC |
Cat# CRL-3216 |
Experimental Models: Organisms/Strains |
C57BL/6 (C57BL/6 NCrL) mice |
Charles River Laboratories |
Cat# C57BL/6NCrl |
|
|
|
|
|
|
|
|
|
|
|
|
Oligonucleotides |
Synthetic RNA poluCUG repeat (5X) |
IDT |
N/A |
Oligo-dT(20) |
Gift from Dr. J. Paul Taylor (St. Jude Children Hospital) |
N/A |
|
|
|
|
|
|
|
|
|
Recombinant DNA |
pDUET-HA-FUSWT
|
This paper |
N/A |
pET His-TDP-43WT
|
This paper |
N/A |
pET His-SOD1A4V
|
This paper |
N/A |
pRK2- His-FUSWT-mCherry |
This paper |
N/A |
pRK2-His-TDP-43WT-mCherry |
This paper |
N/A |
pRK2-His-TDP-43G298S-mCherry |
This paper |
N/A |
pRK2-His-SOD1G93R-mCherry |
This paper |
N/A |
pX330-U6-Chimeric_BB-CBh-hSpCas9 |
Addgene Cat# 42230 |
(Cong et al., 2013) |
Lentivirus |
EF1a-Tet3G-IRES-Neo |
This paper |
N/A |
Ubic-myc-TDP-43WT-mRuby2-SV40-Puro |
This paper |
N/A |
TRE3G-TDP-43ΔNLS(K82A,R83A,K84A)1−414-Clover-EF1acore-BSD |
This paper |
N/A |
Ubic-UBAP2L-mRuby2-SV40-Puro |
This paper |
N/A |
Ubic-TDP-43ΔNLS(K82A,R83A,K84A)1−414-Clover-SV40-Puro |
This paper |
N/A |
pCDH-CMVtet-EYFP-TDP-43 |
This paper |
N/A |
|
|
|
Software and Algorithms |
FIJI (ImageJ) |
NIH |
Fiji, RRID: SCR_002285 https://fiji.sc/#download
|
Benchlin webtool |
Benchling |
https://benchling.com/signup |
Graphpad prism |
Graphpad |
Graphpad Prism RRID:SCR_002798 https://www.graphpad.com/scientific-software/prism/
|
|
|
|
|
|
|
Other |
96 well glass bottom plate |
Cellvis |
Cat# P96–1.5H-N |
8 well chamber slides (glass bottom) |
Ibidi |
Cat# 80827 |
GSTrap HP columns |
GE |
Cat# 17–5281-01 |
HisTrapHP colums |
GE |
Cat# 7–5248-02 |
Superdex 75 10/300 |
GE |
Cat# 17–5174-01 |
Sephadex G-25 in PD-10 |
GE |
Cat# 17–0851-01 |
Hemacytometer |
Fisher Scientific |
Cat# 267110 |
DeltaVision Core system |
GE |
N/A |
Olympus FV1000 Spectral Confocal with SIM Scanner |
Olympus |
N/A |
Tecnai G2 Spirit BioTWIN transmission electron microscope equipped |
Tecai |
N/A |
Zeiss LSM 880 confocal microscope |
Zeiss |
N/A |
CQ1 confocal quantitative image cytometer |
Yokogawa |
N/A |
SH800S Sony cell sorter |
Sony |
N/A |
JEOL 1200 EX II TEM |
JEOL USA |
N/A |
Akta Pure |
GE |
N/A |
Amaxa Nucleofactor |
Lonza |
N/A |
C-B 300 mesh Cu-01813-F |
Ted pella |
Cat# Cu-01813-F |
|
|
|
|
|
|
|
|
|
|
|
|