Fig 4. Induction of SasG expression in S. aureus leads to unclumping, detachment and dissemination.

Clumping (A), adhesion to fibrinogen in 96-well plates (B), and dissemination from an infected plasma clot (C) of S. aureus LAC carrying Tet-inducible SasG expression vectors pRMC2-SasG (A) and pALC2073-SasG (B-C), induced by addition of BHI and 400 ng/ml anhydrotetracycline (aTet). N = 6 per group. *p<0.05, **p<0.01, ****p<0.0001. Data presented as mean ± SEM.