Figure 2.

Emergence of ribozymes and kinetic characteristics. (A) In k-Seq, an RNA pool enriched for active ribozymes is reacted at multiple BYO concentrations, in triplicate. Captured RNA is then reverse-transcribed and sequenced. Activity curves are constructed for sequences detected in the enriched pool. (B) Aminoacylation at various [BYO] for ribozyme S-2.1-a observed by both gel shift and k-Seq Data for all other measured ribozymes are shown in Supporting Figure S3. Error bars correspond to standard deviation among triplicates. (C) Correlation between catalytic enhancement of ten ribozymes, measured by gel shift assay and k-Seq Error bars correspond to standard deviation among triplicates (k-Seq) or 2–3 replicates (gel assay) (R² = 0.87; Supporting Table S1). Dotted orange line indicates line of unity.