Figure 3.
Aminoacylation site and landscape ruggedness. The likely site of BYO modification on ribozyme S-1A.1-a was identified by stalling of reverse transcription, resulting in a truncated product (A). The site, G65, was verified by loss of activity upon 2′-O-methylation, assayed by streptavidin gel shift after BYO reaction (B). 2′-O-Methylation of an adjacent site (C64) did not show loss of activity. (C) Average correlation of fitness effects γd as a function of edit distance d, shown for the sequence families around the five most abundant centers: 2.1 (magenta), 1A.1 (red), 1B.1 (orange), 1B.2 (green), 1A.2 (blue).