Staining for extracellular matrix components is enhanced in chondrocytes cultured in hypoxia. Alginate bead cultures of chondrocytes after 17 days in normoxia (A,C,E) or hypoxia (B,D,F). Sections were stained with Hematoxylin and Eosin (A,B), Safranin O (C,D), or Toluidine Blue (E,F). Calibration bar = 30 microns.