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. Author manuscript; available in PMC: 2020 May 2.
Published in final edited form as: Cell Stem Cell. 2019 Apr 11;24(5):753–768.e6. doi: 10.1016/j.stem.2019.03.010

Fig. 6: Wnt/β-Catenin-active prostate stromal cells suppress basal cell proliferation via Tgfβ See also Figure S6.

Fig. 6:

(A-B) Dot graphs quantify sphere-forming units (A) and sphere sizes (B) derived from prostate epithelial cells co-cultured with and without UGSM cells. Epithelial cells are infected by lentivirus expressing GFP (CGW-control) or dominant negative (DN) TgfβRII, whereas UGSM cells are infected by lentivirus that express RFP (CRW-control) or S37A β-Catenin. Data show means ± s.d. from three independent experiments. (C) Transillumination images of regenerated prostate tissues derived from prostate epithelial cells infected with lentivirus expressing GFP (control) and dominant negative (DN) TgfβRII, respectively. Bar = 5 mm. Dot graph shows means ± s.d. of xenograft weight. N=5. (D) Co-immunostaining of BrdU and K5 in regenerated prostatic tissues. Bars = 50 μm. Dot graph shows means ± s.d. of percentage of BrdU+ epithelial cells in regenerated prostatic tissues. Each dot represents result from one image. Data were collected from 4 pairs of regenerated prostatic tissues. n.s.: not significant.