Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Gene (Exaiptasia pallida) | Aiptasia canonical NPC2 XM_021041171.1 | NA | XM_021041171.1 | |
| Gene (E. pallida) | Aiptasia canonical NPC2 XM_021041174.1 | NA | XM_021041174.1 | |
| Gene (E. pallida) | Aiptasia canonical NPC2 XM_021041173.1 | NA | XM_021041173.1 | |
| Gene (E. pallida) | Aiptasia canonical NPC2 XM_021046710.1 | NA | XM_021046710.1 | |
| Gene (E. pallida) | Aiptasia non-canonical NPC2 XM_021052412.1 | NA | XM_021052412.1 | |
| Gene (E. pallida) | Aiptasia non-canonical NPC2 XM_021052404.1 | NA | XM_021052404.1 | |
| Gene (E. pallida) | Aiptasia non-canonical NPC2 XM_021052381.1 | NA | XM_021052381.1 | |
| Gene (E. pallida) | Aiptasia RPS7 | NA | KXJ15968.1 | |
| Gene (Nematostella vectensis) | Nematostella canonical NPC2 XM_001622824.1 | NA | XM_001622824.1 | |
| Gene (N. vectensis) | Nematostella canonical NPC2 XM_001635452.1 | NA | XM_001635452.1 | |
| Gene (N. vectensis) | Nematostella non-canonical NPC2 XM_001627305.1 | NA | XM_001627305.1 | |
| Gene (N. vectensis) | Nematostella RPS7 | NA | jgi|Nemve1|54581|gw.133.186.1 | |
| Gene (Acropora digitifera) | Acropora canonical NPC2 XM_015898926.1 | NA | XM_015898926.1 | |
| Gene (A. digitifera) | Acropora canonical NPC2 XM_015898919.1 | NA | XM_015898919.1 | |
| Gene (A. digitifera) | Acropora canonical NPC2 XM_015910954.1 | NA | XM_015910954.1 | |
| Gene (A. digitifera) | Acropora canonical NPC2 XM_015898921.1 | NA | XM_015898921.1 | |
| Gene (A. digitifera) | Acropora non-canonical NPC2 XR_001561549.1 | NA | XR_001561549.1 | |
| Gene (A. digitifera) | Acropora non-canonical NPC2 AD_NPC2_6 | NA | AD_NPC2_6 | see Supplementary file 1 |
| Gene (A. digitifera) | Acropora non-canonical NPC2 AD_NPC2_7 | NA | AD_NPC2_7 | see Supplementary file 1 |
| Gene (A. digitifera) | Acropora RPS7 | NA | XM_015898841.1 | |
| Gene (Cladocopium goreaui) | Acropora digitifera resident Symbiodiniaceae symbionts, cp23S marker | NA | MK696595 | |
| Gene (C. goreaui) | CCMP2466 Symbiodiniaceae culture, cp23S marker | NA | MK696599 | |
| Strain, strain background (Exaiptasia pallida)(male) | Aiptasia line CC7 | DOI: 10.1038/srep15677 | in DOI: 10.1186/1471-2164-10-258 | |
| Strain, strain background (E. pallida)(female) | Aiptasia line F003 | DOI: 10.1038/srep15677 | ||
| Strain, strain background (E. pallida)(female) | Aiptasia line H2 | DOI: 10.1038/srep15677 | ||
| Strain, strain background (Nematostella vectensis)(male and female) | Nematostella | Prof. Dr. Thomas Holstein, Heidelberg University | ||
| Strain, strain background (Breviolum minutum) | Symbiodiniaceae strain SSB01 | DOI: 10.1111/jpy.12055 | GenBank:MK692539 | Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Strain, strain background (Symbiodinium linuchae) | Symbiodiniaceae strain SSA01 | DOI: 10.1038/srep15677 | GenBank:MK692538 | Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Strain, strain background (Symbiodinium necroappetens) | Symbiodiniaceae strain SSA02 | DOI: 10.1111/jpy.12055 | GenBank:MK692866 | Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Strain, strain background (Effrenium voratum) | Symbiodiniaceae strain SSE01 | DOI: 10.1111/jpy.12055 | GenBank:MK696597 | Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Strain, strain background (Cladocopium goreaui) | Symbiodiniaceae strain CCMP2466 | National Center for Marine Algae and Microbiota (NCMA), Bigelow Laboratory for Ocean Sciences, Maine, USA | GenBank:MK696600 | Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Strain, strain background (Durusdinium trenchii) | Symbiodiniaceae strain CCMP2556 | National Center for Marine Algae and Microbiota (NCMA), Bigelow Laboratory for Ocean Sciences, Maine, USA | GenBank: MK692915 |
Accession number for rDNA 28S LSU marker (DOI: 10.1016/j.cub.2018.07.008) |
| Cell line (Homo sapiens) | HEK 293T | Deutsche Sammlung von Mikroorganismen und Zellkulturen (DMSZ) from Dr. C. Niehrs and Dr. S. Acebrón | ACC 305 | authenticated by DMSZ, confirmed mycoplasma-free |
| Biological sample (Acropora digitifera) | Acropora digitifera | this paper | Collected off Sesoko Island, Okinawa Prefecture, Japan (26°37'41"N, 127°51'38"E) | |
| Antibody | anti-Aiptasia NPC2 XM_021052412 | this paper | Rabbit polyclonal, 0.3 mg/ml. (Westerns 1:500-1:1000, Dot Blot 1:1000, IF 1:200) BioScience GmbH. | |
| Antibody | anti-Aiptasia NPC2 XM_021052404 | this paper | Rabbit polyclonal, 0.45 mg/ml. (Westerns 1:2000-1:4000, Dot Blot 1:5000, IF 1:100-1:750) BioScience GmbH. | |
| Antibody | anti-Aiptasia NPC2 XM_021052381 | this paper | Rabbit polyclonal, 0.4 mg/ml (Westerns 1:500, Dot Blot 1:500, IF 1:200) BioScience GmbH. | |
| Antibody | HRP-coupled anti-rabbit (goat polyclonal) | Jackson ImmunoResearch | Jackson ImmunoResearch:111-035-144 | (Western blot 1:10,000) |
| Antibody | anti-alpha-tubulin (mouse monoclonal) | Sigma-Aldrich | Sigma-Aldrich:T9026 | (Western blot 1:1000) |
| Antibody | HRP-coupled anti-mouse (goat polyclonal) | Jackson ImmunoResearch | Jackson ImmunoResearch:115-035-044 | (Western blot 1:10,000) |
| Antibody | anti-rabbit IgG-Alexa488 (goat polyclonal) | Abcam | Abcam:ab150089 | (IF 1:500) |
| Antibody | anti-mCherry (rabbit polyclonal) | Thermo Fisher Scientific | Thermo Fisher Scientific:PA5-34974 | (Western blot 1:3000) |
| Antibody | conformation-specific HRP-coupled anti-rabbit IgG (mouse monoclonal) | Cell Signaling Technology | CST:5127S | (Western blot 1:2000) |
| Recombinant DNA reagent | NPC2 XM_021052412 for recombinant expression in HEK293T cells (plasmid) | this paper | NPC2-5P-crmCherry (DOI: 10.1371/journal.pone.0088893)−3xHA (YPYDVPDYA). Progenitors: PCR (cDNA), vector pCEP | |
| Recombinant DNA reagent | NPC2 XM_021052404 for recombinant expression in HEK293T cells (plasmid) | this paper | NPC2-5P-crmCherry (DOI: 10.1371/journal.pone.0088893)−3xHA (YPYDVPDYA). Progenitors: PCR (cDNA), vector pCEP | |
| Recombinant DNA reagent | NPC2 XM_021041171 for recombinant expression in HEK293T cells (plasmid) | this paper | NPC2-5P-crmCherry (DOI: 10.1371/journal.pone.0088893)−3xHA (YPYDVPDYA). Progenitors: PCR (cDNA), vector pCEP | |
| Recombinant DNA reagent | crmCherry:3xHA control for recombinant expression in HEK293T cells (plasmid) | this paper | crmCherry (DOI: 10.1371/journal.pone.0088893)−3xHA (YPYDVPDYA). Progenitors: PCR (cDNA), vector pCEP | |
| Sequence-based reagent | Primers for qPCR of Aiptasia, Acropora, Nematostella NPC2s | this paper | see Supplementary file 3 for all primer sequences | |
| Peptide, recombinant protein | K-YGIDVFCDEIRIHLT | Custom peptide, INTAVIS Bioanalytical Instruments AG | Epitope for antibody against Aiptasia NPC2 XM_021052412 | |
| Peptide, recombinant protein | K-AKNDIFCNSIPFNLV | Custom peptide, INTAVIS Bioanalytical Instruments AG | Epitope for antibody against Aiptasia NPC2 XM_021052404 | |
| Peptide, recombinant protein | K-VQNNVLCGEVTLTLM | Custom peptide, INTAVIS Bioanalytical Instruments AG | Epitope for antibody against Aiptasia NPC2 XM_021052381 | |
| Commercial assay or kit | RNeasy kit | Qiagen | Qiagen:74104 | |
| Commercial assay or kit | SYBR Hi-ROX qPCR master mix | Bioline | BIO-92005 | |
| Commercial assay or kit | NHS-activated Sepharose Fast Flow 4 | GE Health Care Life Sciences | GE Healthcare Sciences:17090601 | |
| Commercial assay or kit | anti-HA magnetic beads | Miltenyi Biotech | Miltenyi Biotech:130-091-122 | |
| Commercial assay or kit | Dynabeads Antibody Coupling Kit | Thermo Fisher Scientific | Thermo Fisher Scientific:14311 | |
| Commercial assay or kit | Pierce BCA Protein Assay Kit | Thermo Fisher Scientific | Thermo Fisher Scientific:23227 | |
| Chemical compound, drug | Trizol | Life Technologies | Life Technologies:15596026 | |
| Chemical compound, drug | MSTFA (N-Methyl-N-(trimethylsilyl)trifluoroacetamide | Sigma-Aldrich | Sigma-Aldrich:69479 | |
| Chemical compound, drug | Lipofectamine2000 | Thermo Fisher Scientific | Thermo Fisher Scientific:11668019 | |
| Chemical compound, drug | Cholesterol-D6 | Cambridge Isotope Laboratories | Cambridge Isotope Laboratories:DLM2607 | |
| Chemical compound, drug | Acetyl chloride in methylene chloride | Sigma-Aldrich | Sigma-Aldrich:708496 | |
| Chemical compound, drug | U18666A | Sigma-Aldrich | Sigma-Aldrich:U3633 | |
| Chemical compound, drug | RNAlater | Thermo Fisher Scientific | Thermo Fisher Scientific:AM7020 | |
| Software, algorithm | Geneious | DOI: 10.1093/bioinformatics/bts199 | v. 9 | |
| Software, algorithm | SignalP 4.0 | DOI: 10.1038/nmeth.1701 | v. 4.0 | |
| Software, algorithm | MEGA | DOI: 10.1093/molbev/msw054 | v. 7.10.8 | |
| Software, algorithm | MrBayes | DOI: 10.1093/bioinformatics/17.8.754 | v. 3.2.6; plugin for Geneious | |
| Software, algorithm | DataMonkey server | DOI: 10.1093/bioinformatics/bti320 | Datamonkey classic server | |
| Software, algorithm | HyPhy program suite | DOI: 10.1093/bioinformatics/bti079 | accessed via DataMonkey classic server |
|
| Software, algorithm | single-likelihood ancestor counting (SLAC) | DOI: 10.1093/molbev/msi105 | accessed via DataMonkey classic server | |
| Software, algorithm | mixed effects models of evolution (MEME) | DOI: 10.1371/journal.pgen.1002764 | accessed via DataMonkey classic server | |
| Software, algorithm | MCMC.qPCR | DOI: 10.1371/journal.pone.0071448 | R library | |
| Software, algorithm | GCMS Postrun Analysis software | Shimadzu | ||
| Software, algorithm | Analyst | SCIEX | v. 1.6.3. Control and analysis software for QTRAP 5500 MS | |
| Software, algorithm | LipidView | SCIEX | v. 1.2 | |
| Software, algorithm | Fiji | DOI: 10.1038/nmeth.2019 | v. 2.0.0-rc-67/1.52d | |
| Other | Phalloidin; Phalloidin-Atto 565 | Sigma-Aldrich | Sigma-Aldrich:94072 | |
| Other | Hoechst; Hoechst 33258 | Sigma-Aldrich | Sigma-Aldrich:B2883 |