Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference | Identifiers | Additional information |
|---|---|---|---|---|
| Cell line (human) | HeLa | ATCC | Cat. #: CCL-2; RRID:CVCL_0030 | |
| Antibody | Mouse monoclonal anti-CHC17 (X22) |
Frances Brodsky PMID: 2415533 | IF (5 mg/mL) | |
| Antibody | Mouse monoclonal anti-CHC17 (TD.1) | Frances Brodsky PMID: 1547490 | WB (1.3 mg/mL) | |
| Antibody | Rabbit polyclonal anti-CHC22 (SHL-KS) | Frances Brodsky PMID: 29097553 | WB (0.4 mg/mL) | |
| Antibody | Mouse monoclonal anti-β-actin (AC-15) | Sigma | Cat. #: A1978; RRID:AB_476692 | WB (1:2000) |
| Antibody | Purified anti-HA.11 (16B12) | Covance | Cat. #: MMS-101P; RRID:AB_10064068 | |
| Antibody | Rabbit polyclonal anti-CHC22 | Proteintech | Cat. #: 22283–1-AP; RRID:AB_11183764 | |
| Antibody | Goat anti-rabbit IgG coupled to HRP | ThermoFisher Scientific | Cat. #: 172–1019 | WB (1:8000) |
| Antibody | Goat anti-mouse IgG coupled to HRP | ThermoFisher Scientific | Cat. #: 170–6516 | WB (1:8000) |
| Antibody | Anti-mouse IgG1 coupled to Brilliant Violet 421 (RMG1-1) | Biolegend | Cat. #: 406616; RRID:AB_2562234 | FC (1:200) |
| Recombinant DNA reagent | HA-GLUT4-mCherry | This paper | Generated from HA- GLUT4-GFP (gift from Dr Tim McGraw, PMID: 11058093) |
|
| Recombinant DNA reagent | CHC22V (pEGFP-C1-GFP-CHC22V) | Frances Brodsky PMID: 20065094 | ||
| Recombinant DNA reagent | CHC22M (pEGFP-C1-GFP-CHC22M) | This paper | Generated by Quick change mutagenesis from CHC22V | |
| Recombinant DNA reagent | CHC17 (pEGFP-C1-GFP-CHC17) | Frances Brodsky PMID: 29097553 | ||
| Sequence-based reagent | AllStars Negative Control siRNA |
Qiagen | Cat. #: SI03650318 | |
| Commercial assay or kit | Quick change mutagenesis | New England Biolabs, USA | Cat. #: E0554S | |
| Commercial assay or kit | BCA | Pierce | Cat. #: 23225 | |
| Commercial assay or kit | Western Lightning Chemilumi-nescence Reagent |
GE Healthcare | Cat. #: RPN2209 | |
| Chemical compound, drug |
JetPrime transfection reagent |
PolyPlus | Cat. #: 114–07 | |
| Chemical compound, drug | Insulin | Sigma | Cat. #: I9278 | |
| Chemical compound, drug |
Bovine serum albumin (BSA) | Sigma | Cat. #: A7906 | |
| Software, algorithm |
FlowJo | Treestar | ||
| Software, algorithm | ImageJ | NIH | ||
| Software, algorithm | Prism | Graphpad | ||
| Software, algorithm | R | R Project | Packages: pegas, Smisc, gplots | |
| Other | CellView glass bottom culture dish | Greiner Bio-one | Cat. #: 627860 | |
| Other | Nitrocellulose membrane |
Biorad | Cat. #: 1620112 |