Key resources table.
| Reagent type (species) or resource |
Designation | Source or reference |
Identifiers | Additional information |
|---|---|---|---|---|
| Genetic reagent (G. gallus) | GFP+/Hyline cross | Roslin Institute | Fertile heterozygous eggs for PGC derivations (Pettersen et al., 2004) | |
| Primary cells (G. gallus) | Primordial Germ Cells (PGCs) | G. gallus GFP+/Hyline cross, Roslin Institute (This study) | ||
| Cell line (G. gallus) | DF-1 fibroblasts | American Type Culture Collection | CRL-12203; RRID:CVCL_0570 | |
| Cell line (C. sapiens) | MDCK | American Type Culture Collection | CCL- 34; RRID:CVCL_0422 | |
| Cell line (H. sapiens) | 293T | American Type Culture Collection | CRL-3216; RRID:CVCL_0063 | |
| Cell line (H. sapiens) | eHAP1 | Horizon Discovery | C669 | |
| Antibody | rabbit polyclonal α-ANP32A | Sigma-Aldrich | AV40203; RRID:AB_1844874 | Dilution 1:500-1:1000 |
| Antibody | mouse monoclonal α-β-actin | Sigma-Aldrich | A2228; RRID:AB_476697 | Dilution 1:1000 |
| Antibody | mouse monoclonal α-FLAG | Sigma-Aldrich | F1804; RRID:AB_262044 | Dilution 1:1000 (WB), 1:300 (IF) |
| Antibody | mouse monoclonal α-Lamin B1 | Merck | MAB5492; RRID:AB_2085944 | Dilution 1:1000 |
| Antibody | mouse monoclonal α-PCNA | Santa Cruz | sc-25280, RRID:AB_628109 | Dilution 1:1000 |
| Antibody | rabbit polyclonal α-Histone 3 | Abcam | AB1791; RRID:AB_302613 | Dilution 1:2000 |
| Antibody | rabbit monoclonal α-vinculin | Abcam | AB129002; RRID:AB_11144129 | Dilution 1:1000 |
| Antibody | rabbit polyclonal α-Gaussia Luc | NEB | E80235 | Dilution 1:2000 |
| Antibody | rabbit polyclonal α-PB1 | Invitrogen | PA5-34914; RRID:AB_2552264 | Dilution 1:2000 |
| Antibody | rabbit polyclonal α-PB2 | GeneTex | GTX125926; RRID:AB_11162999 | Dilution 1:2000 |
| Antibody | goat polyclonal anti-rabbit HRP | CST | 7074 | Dilution 1:2000 |
| Antibody | Horse polyclonal anti-mouse HRP | CST | 7076 | Dilution 1:2000 |
| Antibody | Sheep polyclonal α-rabbit HRP | Merck | AP510P | Dilution 1:20000 |
| Antibody | goat polyclonal α-mouse HRP | AbD Serotec | STAR117P; RRID:AB_323839 | Dilution 1:10000 |
| Antibody | goat polyconal α-mouse AlexaFluor-568 | Invitrogen | A11031; RRID:AB_144696 | Dilution 1:1000 |
| Recombinant DNA reagent | pGEM-T Easy vector | Promega | A1360 | |
| Recombinant DNA reagent | pSpCas9(BB)−2A-Puro PX459 V2.0 vector | Gift from Dr. Feng Zhang | RRID:Addgene_62988 | |
| Recombinant DNA reagent | pSpCas9n(BB)−2A-GFP PX461 vecotr | addgene | Plasmid 48140; RRID:Addgene_48140 | |
| Recombinant DNA reagent | pCAGGS vector | Belgium Co-ordinated Collections of Microorganisms (BCCM), University of Ghent, Belgium | ||
| Recombinant DNA reagent | H5N1 A/turkey/England/50-92/1991 polI plasmids | APHA, Weybridge, UK | ||
| Recombinant DNA reagent | H7N9 Anhui/1/2013 pHW2000 plasmids | Pirbright Institute, UK | ||
| Recombinant DNA reagent | H1N1 A/PR/8/34 (PR8) polI or pHW2000 plasmids | (Neumann et al., 1999) | ||
| Commercial assay or kit | RNeasy mini kit | Qiagen | 74106 | |
| Commercial assay or kit | Quick Start Bradford Protein Assay Kit | Biorad | 5000202 | |
| Commercial assay or kit | Dual-luciferase Reporter assay system | Promega | E1910 | |
| Commercial assay or kit | Renilla luciferase kit | Promega | E2810 | |
| Commercial assay or kit |
QIAamp Viral RNA Mini Kit | Qiagen | 52906 | |
| Chemical compound, drug | Lipofectamine 3000 | Invitrogen | L3000008 | |
| Chemical compound, drug |
Lipofectamine 2000 | Invitrogen | 11668019 | |
| Software, algorithm | Image J | ImageJ (http://imagej.nih.gov/ij/) | ||
| Software, algorithm | GraphPad Prism | GraphPad Prism (https://graphpad.com) | version 6 | |
| Software, algorithm | Geneious | Geneious https://www.geneious.com | R6 |