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. 2019 Jun 4;8:e44927. doi: 10.7554/eLife.44927

Figure 2. Gene expression control using OF mice to target different cell types within the same brain region.

Figure 2.

(A) Schematic showing Cre (left) and Flp (right) recombinase-dependent gene expression control. (B and E) The breeding scheme for Orexin-Flp; MCH-Cre and Orexin-Flp; Gad67-Cre bigenic mice, respectively. (C and F) Schematic drawings showing micro-injection of the AAV cocktail into the LHA of bigenic mice. (D and G) Representative coronal brain sections showing the segregated expression of tdTomato and hrGFP in a Cre and Flp recombinase-dependent manner, respectively.