| Component | Amount per reaction | Final concentration |
|---|---|---|
| pU6-SAG1 | 400 μg | 1 μg/μl |
| NEB Buffer 3.1 | 40 μl | 1x |
| AseI | 400 U | 1 U/μl |
| UltraPure water | To 400 μl |
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