| Component | Amount per reaction | Final concentration |
|---|---|---|
| 2X Q5 master mix | 50 μl | 1x |
| Primer 1 (10 μM) | 5 μl | 0.5 μM |
| Primer 2 (10 μM) | 5 μl | 0.5 μM |
| Template DNA | 1 μg of genomic DNA or 50 ng of library DNA | |
| UltraPure water | To 100 μl |
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