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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Anal Biochem. 2019 Apr 11;577:1–13. doi: 10.1016/j.ab.2019.04.009

Figure 3. Internal standards for quantitation of PLA spots relative to cell density or LC20/LC20 isPLA.

Figure 3.

Representative traces of the force generated by isometric contraction of murine gastric fundus smooth muscle strips in response to 1μM CCh (A), or 10Hz EFS (B). Arrowheads indicate the time point at which the strips were submerged into room temperature 4% (w/v) PFA. Representative images of β1integrin immunofluorescence from gastric fundus smooth muscles that were unstimulated (Control, n=3) (C), contracted by 1μM CCh, n=3 (D), or 10Hz EFS, n=3 (E). Representative images of LC20/LC20 PLA from unstimulated gastric fundus smooth muscles (Control, n=3) (F), contracted by 1μM CCh, n=3 (G), or 10Hz EFS, n=3 (H). (I) Average ± SD of the cellular cross-sectional areas. (J) Average ± SD of the number of PLA spots per total image area. Cell nuclei were labeled with DAPI (blue).