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. Author manuscript; available in PMC: 2020 Jul 15.
Published in final edited form as: Anal Biochem. 2019 Apr 11;577:1–13. doi: 10.1016/j.ab.2019.04.009

Figure 4. MYPT1, CPI-17, and LC20 phosphorylation in response to cholinergic stimulation of gastric fundus muscle strips.

Figure 4.

Representative isPLA images and average ratios ± SD of MYPT1/pT853 (A, B), MYPT1/pT696 (C, D), CPI-17/pT38 (E, F), and LC20/pS19 (G, H) from muscles with no treatment (Control), 1μM CCh, 1min, in the presence of 0.3μM tetrodotoxin, and 10Hz, 5sec EFS in the presence of 100μM L-NNA and 1μM MRS2500. ***P<0.001, ****P<0.0001, ns P>0.05, n=3 for each treatment.