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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Res. 2019 Apr 5;79(11):2962–2977. doi: 10.1158/0008-5472.CAN-18-3151

Figure 1: Large microtumors exhibit hypoxia, collective migration and aggressive phenotype.

Figure 1:

(A) Schematic defining distance of migration (d), area of microtumor migrated out of the microwell (AMigration) and total area of the microtumor (ATotal); (B) Distance of migration for individual microtumors; each dot represents data for individual microtumor. (C) Migration trajectories of individual 600μm microtumors from day 1 to day 6 showing inter-tumoral variation in migration kinetics; Extent of migration for each time point was calculated as migrated distance/the total area of microtumor X 100. Levels of sE-CAD (D, ELISA) and sE-CAD, FN, Pro-MMP9 and MMP9 (E, western blot) in CM; (F) pERK expression in microtumor cell lysates. Data presented is representative from three independent experiments (n=3). ***p<0.001, p****< 0.0001; Unpaired t-test.