Key Resources Table
| Reagent type | Designation | Source | Identifier | Monoclonal (M) or Polyclonal (P) | Host & Sub-class | Clone name |
|---|---|---|---|---|---|---|
| Antibody | Anti-human TMIGD2/CD28H | R&D Systems | MAB83162 | M | Mouse IgG2b | 953743 |
| Antibody | Anti-human TMIGD2/CD28H AF647 | R&D Systems | FAB83162R | M | Mouse IgG2b | 953743 |
| Antibody | Anti-human 2B4 | BioLegend | 329502 | M | Mouse IgG1 | C1.7 |
| Antibody | Anti-human NKG2D | R&D Systems | MAB139 | M | Mouse IgG1 | 149810 |
| Antibody | Anti-human NKp46 | BD Biosciences | 557847 | M | Mouse IgG1 | 9-E2 |
| Antibody | Anti-human CD2 | BD Biosciences | 555323 | M | Mouse IgG1 | RPA-2.10 |
| Antibody | Anti-human DNAM-1 | BD Biosciences | 559787 | M | Mouse IgG1 | DX11 |
| Antibody | Anti-human CD20 hIgG1 chimera (Rituximab) |
Genentech | M | |||
| Antibody | Anti-human CD107a PE | BD Biosciences | 555801 | M | Mouse IgG1 | H4A3 |
| Antibody | Anti-human CD107a FITC | BD Biosciences | 555800 | M | Mouse IgG1 | H4A3 |
| Antibody | Anti-human CD56 BV421 | BD Biosciences | 562751 | M | Mouse IgG2b | NCAM16.2 |
| Antibody | Goat anti-mouse F(ab’)2 PE | Jackson ImmunoResearch | 115–116-071 | P | Goat | |
| Antibody | Anti-human CD16 | BD Biosciences | 555404 | M | Mouse IgG1 | 3G8 |
| Antibody | Anti-human CD56 | BD Biosciences | 555513 | M | Mouse IgG1 | B159 |
| Antibody | Anti-human B7H7 | R&D Systems | MAB80841 | M | Mouse IgG1 | 907812 |
| Antibody | Anti-human B7H7 AF647 | R&D Systems | FAB80841R | M | Mouse IgG1 | 907812 |
| Antibody | Anti-human HLA-E (3D12) | Gift from D. Geraghty (Fred Hutchinson Cancer Research Center, Seattle) |
M | Mouse IgG1 | 3D12 | |
| Antibody | Anti-human NKG2A (Z199) | Beckman Coulter | IM2751 | M | Mouse IgG2b | Z199 |
| Antibody | Anti-human IFNγ APC | BioLegend | 506510 | M | Mouse IgG1 | B27 |
| Antibody | Anti-human TNFα BV650 | BioLegend | 502937 | M | Mouse IgG1 |
MAb11 |
| Antibody | Anti-human MIP-1α FITC | Invitrogen | MA523564 | M | Mouse IgG2b | 93342 |
| Antibody | Anti-human MIP-1β PerCP-Cy5.5 | BD Biosciences | 560688 | M | Mouse IgG1 | D21–1351 |
| Antibody | Anti-2A peptide | Millipore | ABS31 | P | Rabbit | |
| Antibody | Anti-phosphotyrosine (4G10) | Millipore (Upstate) | 05–321 | M | Mouse IgG2b | 4G10 |
| Chemical compound | Near-IR Dead Cell Stain Kit | Thermo Fisher Scientific | L34975 | |||
| Chemical compound | PKH67 Green Fluorescent Cell Linker Kit for General Cell Membrane Labeling |
SIGMA Aldrich |
PKH67GL | |||
| Commercial kit | In-Fusion HD cloning kit | Clontech Laboratories | 639648 | |||
| Commercial kit | ToxiLight Non-Destructive Cytotoxicity BioAssay Kit | Lonza | LT07–117 | |||
| Commercial kit | EasySep Human NK Cell Enrichment Kit | STEMCELL Technologies | 19055 |