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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Cancer Res. 2019 Mar 1;17(6):1253–1263. doi: 10.1158/1541-7786.MCR-18-1054

Figure 5. SULT2B1b regulates sensitivity to TNF-mediated cell death in LNCaP and C4-2 cells.

Figure 5.

LNCaP (A) or C4-2 (B) cells were transfected with Control or SULT2B1b siRNA prior to addition of indicated concentrations of TNF. All cells were harvested 72 hours later and prepared for cell cycle analysis by flow cytometry. Bars indicate the average percent sub-G1 nuclei +/− SEM of triplicate wells. For all TNF-treated cells in (A) and (B), SULT2B1b KD significantly enhances sub-G1 nuclei percentage over Control KD cells in both LNCaP and C4-2 cells by two-way ANOVA (p<0.0001). Graphs are representative of three independent experiments. (C-D) SULT2B1b expression was evaluated by (C) qRT-PCR and (D) western blot in LNCaP-SULT2B1b cells. (E) LNCaP-SULT2B1b cells were treated with doxycycline prior to addition of indicated concentrations of TNF. After 72 hours, all cells were harvested and prepared for cell cycle analysis by flow cytometry. Bars indicate the mean of % sub-G1 nuclei +/− SEM of triplicate wells evaluated by two-way ANOVA with multiple comparisons test. The graph is representative of duplicate experiments.