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. Author manuscript; available in PMC: 2019 Dec 1.
Published in final edited form as: Mol Cancer Res. 2019 Mar 12;17(6):1391–1402. doi: 10.1158/1541-7786.MCR-18-0763

Figure 4. Ectopic expression of E-cadherin in sarcoma cells inhibits phospho-CREB levels.

Figure 4.

A. A phospho-kinase array revealed that E-cadherin led to downregulation of phospho-CREB. B-C. E-cadherin-mediated phospho-CREB inhibition was verified by B. ELISAs and C. western blotting. D. Abrams canine osteosarcoma cells exhibited reduced phospho-CREB in E-cadherin over-expressing cells. E. QRT-PCR confirmed knockdown of CREB with two independent siRNAs. F. Western blotting to confirm knockdown of CREB in 143B cells. G. CREB knockdown led to a modest downregulation of 143B colony growth in soft agar.