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Photolabeling with PRPs 8–15 in live SET-2 cells. Labeling with (a) hydroxamate PRPs 8–12 and TFMO PRP 15, (b) o-aminoanilide PRPs 13 and 14. In each of (a) and (b), vehicle DMSO (–) and 16 were included as controls for nonspecific bands. (c) competition of 14 HDAC-labeled bands with the HDACi 6. (d) concentration dependent labeling of HDACs by 14. In (c) and (d), densitometry of HDAC2 labeling bands normalized to HDAC2/GAPDH signal is shown to the right of each gel.
