Figure 1: E. coli is both the initiation adder and division adder, robust to static inhibition of biosynthesis.

(A) Division adder vs. initiation adder. Upper: Δ_d is the added size between birth size S_b and division size S_d, and Δ_i is the total added size between two consecutive initiations. s_i is the cell size at initiation per origin, and δ_i is Δ_i per origin. Cell length is used as a proxy for cell size because cell width remains mostly constant during cell elongation [3] (Figure S4). Lower: Illustration of the replication cycle with two overlapping cell cycles.

(B) Resolving overlapping foci using intensity weighting (STAR Methods).

(C) Three major measured physiological parameters show 8%−20% of variation. Each dot represents measurement from a single cell.

(D) Under steady-state growth, E. coli is a division and an initiation adder, with or without static biosynthetic inhibition. Symbols are the binned data and error bars indicate standard errors of mean. In the correlation plots, the variables were rescaled by their means. 6 μM chloramphenicol and 0.05 μg/ml fosfomycin were used. See sample size in Table S3.

See also Figures S1 and S2, and Data S1.