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. Author manuscript; available in PMC: 2020 Jun 3.
Published in final edited form as: Curr Biol. 2019 May 16;29(11):1760–1770.e7. doi: 10.1016/j.cub.2019.04.062

Figure 7: Restoring the division adder.

Figure 7:

(A) Our hypothesis for why E. coli under slow growth conditions deviated from the adder towards the sizer reported in [19]. In slow-growing cells, significant amount of FtsZ is actively degraded by ClpXP [64, 65], which decreases autocorrelations of FtsZ concentration.

(B) We were able to restore the adder in slow growth conditions (doubling time ≈ 4 hours) by repressing clpX expression via tCRISPRi (STAR Methods), confirming our hypothesis. Inset shows that wildtype E. coli is an initiation adder in slow growth conditions. Each shaded area represents the 95% confidence interval of linear fit to the respective raw scatter plot.

See also Figure S7 and STAR Methods.