Figure 1. TILs grown from Patient 1 recognized tumor-specific CTBP1^Q277R mutation.

(A) Four TILs (F1–4) were co-cultured with autologous EBV-B cells electroporated with 11 TMGs encoding tumor-specific mutations, or with sterile water alone (Mock). Following an overnight incubation, IFN-γ concentration in co-culture supernatants was determined by IFN-γ ELISA. (B and C) F3 TILs were co-cultured with EBV-B cells pulsed overnight with individual 25-mer peptides encoded in TMG2, followed by IFN-γ ELISA (B) and with wild-type and mutant CTBP1 25-mer, followed by a multiplex assay for IFN-γ, TNF-α, IL-4, IL-5, IL-10, IL-13 and GM-CSF (C). All the experiments were performed once owing to a limited number of available TILs. Bars represent average reads from two duplicate wells; error bars represent SD.