. Author manuscript; available in PMC: 2020 Aug 5.

Published in final edited form as: J Pharm Biomed Anal. 2019 Apr 15;172:26–32. doi: 10.1016/j.jpba.2019.04.027

Table 1.

Assay performance data for the quantitation of LLOQ, QCL, QCM and QCH of PTX and metabolite concentrations in human plasma.

Analyte	Concentration (ng/mL)	Accuracy (%)	Intra-assay precision (%)	Inter-assay precision (%)
PTX	10 (LLOQ)	109.9	3.5	3.0
	20 (QCL)	110.4	2.0	1.2
	500 (QCM)	102.3	4.0	6.4
	8000 (QCH)	94.3	4.9	7.6
3-p-OH-PTX	1 (LLOQ)	101.2	9.2	NA^*
	2 (QCL)	104.7	4.0	3.2
	50 (QCM)	100.1	2.6	11.3
	800 (QCH)	95.1	3.1	7.0
6-a-OH-PTX	1 (LLOQ)	112.8	7.3	1.9
	2 (QCL)	109.1	3.9	1.1
	50 (QCM)	100.5	1.7	5.8
	800 (QCH)	100.7	3.2	5.0

N=15; 5-fold results, each in 3 separate runs, for each concentration.

The mean square of the within runs was greater than the mean square of the between runs, indicating that there was no significant additional variation due to the performance of the assay in different runs [6].