Table 2.
Multiplex Assay Analytical Validation
Intra-assay Precision (%CV) | Inter-assay Precision (%CV) | LOD | Units | |||||||
---|---|---|---|---|---|---|---|---|---|---|
Low | Medium | High | Avg | Low | Med | High | Avg | |||
CA125 | 3.6% | 5.0% | 10.6% | 7.4% | 9.2% | 4.7% | 3.9% | 5.9% | 0.27 | U/mL |
HE4 | 5.9% | 21.0% | 5.4% | 9.3% | 9.2% | 9.6% | 6.4% | 8.4% | 0.3 | U/mL |
MMP-7 | 2.5% | 8.2% | 9.4% | 7.7% | 4.2% | 6.8% | 4.2% | 7.8% | 249 | pg/mL |
CA72-4 | 3.3% | 14.0% | 7.6% | 8.3% | 53.1% | 15.4% | 13.7% | 27.4% | 203 | pg/mL |