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. Author manuscript; available in PMC: 2020 Jun 1.
Published in final edited form as: Cancer Res. 2019 Apr 17;79(11):2923–2932. doi: 10.1158/0008-5472.CAN-18-2540

Figure 3. PDI KD induces ER stress that downregulates RAD51.

Figure 3.

qPCR (A-B) and Western blot (C) analysis for PDI and RAD51 expression level after doxycycline induction as indicated. qPCR data are presented as means ± S.D. *, p<0.05; **, p<0.01; ***, p<0.001 from three independent experiments. (D) Immunoblot analysis of CRISPR/Cas9 targeting P4HB U87 cells after 48hrs of infection. (E) Tunicamycin (Tm) was added at 5 μg/ul to D54MG and U87MG cells for 12 hrs and RAD51 and BiP protein expression were assessed.