Skip to main content
. Author manuscript; available in PMC: 2020 Jun 11.
Published in final edited form as: Langmuir. 2019 Jan 9;35(23):7354–7363. doi: 10.1021/acs.langmuir.8b02831

Figure 3.

Figure 3.

Characterization of the bulk droplet vitrification method: droplet size, cooling rate, and cryoprotectant agent (CPA) loading. a. Droplet size measurements. Top: picture of vitrified droplets which do not contain hepatocytes. Middle: Image after particle analysis in ImageJ. The small circles correspond to the droplet circumferences. Below: Droplet size distribution b. Droplet temperature after exposure to liquid nitrogen at t=0 seconds. c. Relative volume change of hepatocytes during CPA incubation with exposure to 7.5% (v/v) dimethyl sulfoxide (DMSO) from 0–3 minutes, 15% (v/v) DMSO from 3–6 minutes and 40% (v/v) DMSO with 400mM sucrose from 6–7 minutes. d. Intracellular DMSO concentration during CPA incubation. Scale bars: 25 mm. Error bars: SD.