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. Author manuscript; available in PMC: 2020 Jun 11.
Published in final edited form as: Langmuir. 2019 Jan 9;35(23):7354–7363. doi: 10.1021/acs.langmuir.8b02831

Table 1:

Metabolic activity of fresh, cryopreserved and bulk droplet vitrified hepatocytes in long-term collagen sandwich cultures.

Fresh Cryo Vitrified P values
Mean ± SD Mean ± SD Mean ± SD Fresh-Cryo Fresh-Vitrified Cryo-Vitrified
Presto Blue Day 3 1152 ± 241 565 ± 213 643 ± 271 <0.001 <0.001 0.660
Units: RFU Day 5 787 ± 200 541 ± 319 696 ± 339 0.033 0.569 0.214
Day 7 647 ± 76 255 ± 46 341 ± 107 0.001 0.008 0.606
Urea production Day 2 7.2 ± 1.2 3.6 ± 1.5 4.2 ± 1.4 <0.001 <0.001 0.693
Units: μg/1e6 hepatocytes/24hr Day 3 7.1 ± 1.3 3.1 ± 1.1 4.5 ± 1.1 <0.001 0.001 0.122
Day 4 5.5 ± 2.6 3.6 ± 1.0 4.5 ± 1.3 0.007 0.271 0.232
Day 5 5.4 ± 2.4 3.0 ± 1.8 6.0 ± 1.4 0.002 0.665 <0.001
Day 6 5.8 ± 3.1 2.3 ± 0.8 4.8 ± 2.2 <0.001 0.326 0.002
Day 7 6.8 ± 2.5 2.9 ± 2.0 4.9 ± 1.8 <0.001 0.019 0.013
Albumin synthesis Day 2 0.65 ± 0.30 0.27 ± 0.14 0.45 ± 0.18 <0.001 0.019 0.045
Units: μg/1e6 hepatocytes/24hr Day 3 0.74 ± 0.32 0.24 ± 0.13 0.46 ± 0.01 <0.001 0.001 0.011
Day 4 0.75 ± 0.33 0.28 ± 0.12 0.51 ± 0.12 <0.001 0.006 0.008
Day 5 0.75 ± 0.36 0.31 ± 0.19 0.64 ± 0.07 <0.001 0.284 <0.001
Day 6 0.84 ± 0.31 0.27 ± 0.22 0.62 ± 0.29 <0.001 0.011 <0.001
Day 7 1.11 ± 0.19 0.36 ± 0.25 0.69 ± 0.21 <0.001 <0.001 <0.001

Note. Cryo = cryopreserved; Vitrified = bulk droplet vitrified; RFU = relative fluorescence units.