Table 3.
Characteristics of microsatellite markers used in the study
| Chromosomal location | Nucleotide sequence of the microsatellite marker (5′–3′) | Annealing temperature (°C) | |
|---|---|---|---|
| Forward | Reverse | ||
| 1p31.2 | |||
| D1S2137 | ACATCTTTGGTTTGGATAGATG | CAAAACTGCACATTTTGCAC | 55 |
| D1S368 | GGGCATTGTTTAGGGGTG | TAGTGGGCTTTACGTCTGC | 53 |
| 3p21.3 | |||
| D3S3615 | CCAGGGTCTGTGTGAATGTG | CCCACAGGAGGCATTCAG | 47 |
| 3p24.2 | |||
| D3S1583 | AGCTTGTAAATAGGTCCTAACAGAG | TGGTTTAATAGGCACCGTTT | 58 |
| 9p21.3 | |||
| D9S974 | GAGCCTGGTCTGGATCATAA | AAGCTTACAGAACCAGACAG | 55.5 |
| D9S1604 | CCTGGGTCTCCAATTTGTCA | AGCACATGACACTGTGTGTG | 55.5 |
| 11p15.5 | |||
| D11S4088 | GGGCAGAGGCAGTGGAG | GCATGTTTCGGGGGTG | 55.5 |
| D11S1318 | CCCGTATGGCAACAGG | TGTGCATGTNCATGAGTG | 47 |
| 16q22.1 | |||
| D16S496 | GAAAGGCTACTTCATAGATGGCAAT | ATAAGCCACTGCGCCCAT | 54 |
| D16S3025 | TCCATTGGACTTATAACCATG | AGCTGAGAGACATCTGGG | 45 |