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. 2019 May 29;10:338. doi: 10.3389/fendo.2019.00338

Figure 2.

Figure 2

Energy homeostasis of ARHGAP21 Het mice. VO2 (A) n = 3, heat rate (B) n = 3 and respiratory exchange ratio (RER) (C) n = 3. Ambulatory activity for 24 h during light and dark periods (D) and mean of light and dark periods (E) n = 3. BAT weight (F) n = 6. Real-time PCR assay of ARHGAP21 and UCP1 mRNA levels in BAT (G) n = 3. Brown adipose tissue UCP1 protein content (H), n = 3. The samples were transferred to nitrocellulose membranes in this sequence: Ctl, Het, Ctl, Het, Ctl, and Het. Control mice (Ctl) and ARHGAP21-haplodeficient mice (Het) fed a chow diet for 10 weeks. Data are the mean ± SEM. *P ≤ 0.05 (Student's-t-Test).