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. 2019 May 29;10:338. doi: 10.3389/fendo.2019.00338

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Copyright © 2019 Soares, Zangerolamo, Costa-Júnior, Vettorazzi, Carneiro, Saad, Boschero and Barbosa-Sampaio.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Energy homeostasis of ARHGAP21 Het-HFD mice. VO₂ (A) n = 3, heat rate (B) n = 3, and RER (C) n = 3. Ambulatory activity for 24 h during light and dark periods (D) and mean of light and dark periods (E) n = 3. BAT weight (F) n = 6. Real-time PCR assay of ARHGAP21 and UCP1 mRNA levels in BAT (G) n = 4. Brown adipose tissue UCP1 protein content (H), n = 3–4. The samples were transferred to nitrocellulose membranes in this sequence: Ctl-HFD, Het-HFD, Ctl-HFD, Het-HFD, Ctl-HFD, Het-HFD, and Het-HFD. Control mice (Ctl) and ARHGAP21-haplodeficient mice (Het) fed a high-fat diet for 10 weeks. Data are the mean ± SEM. *P ≤ 0.05 (Student's-t-Test).