Table 1.
Primers sequence and accession number in NCBI.
| Primer | Primer sequence 5′-3′ | Size (bp) | NCBI Accession No. | References |
|---|---|---|---|---|
| CsAMT1 | TTCTCTATCAGTGGGCTTTCG | 141 | AY642427 | This study |
| AGAACCAATGGGACACAACC | ||||
| CsAMT3 | AAGGTAGACGACACAATGG | 109 | XM_004138819 | This study |
| CGTAGAAGATGTTGTTGAGG | ||||
| CsNRT 1.3 | CACAAGCCTTCAGAGAATTGG | 131 | JX206800 | This study |
| TCAACCAGAAAGCACTTATACG | ||||
| CsNR1 | GCACAACTCAGACCAATCC | 103 | HM755943 | This study |
| GATGAGAATGCTGTCCATACC | ||||
| CsNR2 | TGTGCGTGTATTCAGATTCG | 132 | HM755944 | This study |
| GTGCTAGAGGGCGTATAGG | ||||
| CsNiR | AGGATTGGTAGCTTGCACTGG | 105 | EF397679 | This study |
| ACTGTGACTCGCCGTTGC | ||||
| CsGS1 | ATGAGGGAAGAAGGAGGTTACG | 147 | JQ277263 | This study |
| AGAGAAGGTGTGGATGTCAGC | ||||
| CsGOGAT | GGCTGCTCAAGGAAAGGAACC | 127 | DQ641082 | This study |
| TGCTGGATTTGTCACCTGTGC | ||||
| TaAMT1.1 | ACAGCTTCTTCCTCTTCC | 105 | AY525637 | This study |
| CCGAGTAGATGAGGTAGG | ||||
| TaNRT1.1 | ATGCCAGGTTGTCATTGC | 135 | AY587265 | This study |
| CCGAGTCCAGTTGTATGC | ||||
| TaNRT2.1 | TGGACTCCGAGCACAAGG | 104 | AF288688 | This study |
| GACGAAGCAGGTGAAGAAGG | ||||
| TaNRT2.3 | TGGTCAGAGGAGGAGAAGG | 101 | AY053452 | This study |
| GTGGCGAGGATAACATTGC | ||||
| TaNR | ATACACCATGAAAGGATACG | 126 | KY244026 | This study |
| TACTTGTTCGGCTTCTCC | ||||
| TaNiR | CTACACCAACCTCCTCTCC | 138 | FJ527909 | This study |
| GCCAGGTCGTTGATATGC | ||||
| TaGS1 | CCTTGTCATGTGCGATTGC | 135 | DQ124211 | This study |
| GTGTACTCCTGCTCGATACC | ||||
| Ta GOGAT | AAACCAAGGGACCTCAGTATTC | 150 | DW986179 | This study |
| AATGACCACCACCTTCTTACC | ||||
| ZmAMT1;1a | CATCGTCGGAAGGTGTGG | 109 | GRMZM2G175140 | This study |
| TTGGATGATGAGCAGTGACC | ||||
| ZmAMT1;1b | CTACGACTTCTTCCTATACC | 111 | GRMZM2G118950 | This study |
| CGGAGTAGATGAGGTAGG | ||||
| ZmNrt2.1 | TGGACTCAGAGCACAAGG | 102 | AY129953 | This study |
| CGAAGCAGGTGAAGAAGG | ||||
| ZmNR | GCCAGCATTGAAGGGAAG | 106 | M77792 | This study |
| GCTCGTTCTTGAAGTAGACC | ||||
| ZmGS1-3 | CTTGTGATGTGCGATTGC | 129 | X65928 | This study |
| CTCCTGCTCAATACCATACC | ||||
| ZmGS1-4 | AGGCATCAACATCAGTGG | 117 | X65929 | This study |
| AGAATGTAGCGAGCAACC | ||||
| Zm GOGAT | CGCTCTTCTGGCAACTGG | 133 | M59190 | This study |
| CACCTTCCTGTAGTCTGATTCG | ||||
| CsACTIN | AGAGATGGCTGGAATAGAAC | 333 | DQ641117 | Wei et al. (2015) |
| CTGGTGATGGTGTGAGTC | ||||
| ZmACTIN | CATGGAGAACTGGCATCACACCTT | 118 | J01238.1 | Galli et al. (2013) |
| CTGCGTCATTTTCTCTCTGTTGGC | ||||
| TaACTIN | GTCGGTGAAGGGGACTTACA | 187 | AB181991.1 | Moloudi et al. (2013) |
| TTCATACAGCAGGCAAGCAC | ||||
| nifB | GAAGGTGAGAGTGAGGATGG | 88 | MH202771 | This study |
| TTGCTTCAGGCTCATCTCC | ||||
| nifH | GCAACAGTCGGAATACGG | 136 | MH555146 | This study |
| TTGGGTCACGGTCATACG | ||||