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. 2019 May 29;10:654. doi: 10.3389/fphys.2019.00654

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Copyright © 2019 Fiorotti, Menna-Barreto, Gôlo, Coutinho-Rodrigues, Bitencourt, Spadacci-Morena, Angelo and Bittencourt.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Emission spectra obtained by Energy Dispersive X-Ray Spectroscopy (X-EDS) in TEM performed on 3 μm sections of (A) granulocytes from tween-inoculated ticks and (B) granulocytes from fungus-infected ticks. Spectra obtained focusing the incident electron beam on the granulocyte electron-dense granules of which characteristic energies of non-X-ray emission are evident. Multiple X-EDS analyses have been performed in each group; the ones that exhibited a pattern in common with the rest of the acquired spectra are shown. It is also possible to observe the presence of copper (Cu), a component of the TEM supporting grid; osmium (Os), used as fixative; lead (Pb), and uranium (U) that are used as staining.