Skip to main content
. 2019 May 29;10:721. doi: 10.3389/fpls.2019.00721

FIGURE 2.

FIGURE 2

(A) Western blots of 8 μg of gliadins from Perico, THA1, THA7, THA53, BW2003, and BW208 wheat cultivars, incubated with 2 μg of each barley CysProt for 0, 1, and 12 h at 28 °C. Gliadin incubation with HvPap-16+P (A–F); HvPap-1+P (G–L); HvPap-4+P (M–R); HvPap-6 (S–X). (B) Gliadins stability assays after 12 h of incubation without pepsin as control and gliadin treatments with 6 μg of pepsin for 12 h (A–F). Immunoblotting was performed with Gluten Tox G12 HRP-conjugate antibody, 200× (Biomedal diagnostics) following the manufacturer instructions. Pepsin (P) and molecular weight in kilo Dalton unit (kDa).