Figure 5.

MHC-I is not retained in the ER during IBV infection. (A) Outline of experimental design. (B) Autoradiograms of Endo-H sensitivity of immunoprecipitated (IP) MHC-I (w6/32) from C1R-A2 cells, which were either infected with IBV for 16 h or mock-treated, and then metabolically radiolabelled with [³⁵S]methionine and [³⁵S]cysteine and chased for the indicated timepoints. NMS: immunoprecipitated obtained with normal mouse serum as a control. R: Endo-H resistant MHC-I. S: Endo-H sensitive MHC-I. (C) Percent of Endo-H sensitive MHC-I in mock or IBV-treated cells, based on “R” band intensity at each timepoint compare to Endo-H untreated band intensity (columns 2 or 7). (D) Fractions of Endo-H sensitive/resistant MHC-I in mock or IBV-infected cells, based on S/R band intensity relative to their sum for each timepoint. (B–D) Data are representative of two independent experiments.