Skip to main content
. Author manuscript; available in PMC: 2019 Oct 1.
Published in final edited form as: Nat Protoc. 2019 Mar 25;14(4):1261–1279. doi: 10.1038/s41596-019-0141-y

Figure 3. ENC spheroid culture.

Figure 3

a) Protocol (days 12–15) for ENC spheroid formation. NB+N2+B27; NB/N2/B27, Neurobasal medium with N2 and B27 supplement; FGF2, Recombinant Human FGF Basic; CHIR, CHIR 99021. b) Phase contrast and SOX10::GFP reporter line GFP expression of 3D spheroids on day 14. Scale bar = 200 μm.