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. 2019 May 29;9:180. doi: 10.3389/fcimb.2019.00180

Figure 3.

Figure 3

Effect of tipifarnib on invasion of matrigel by E. histolytica. E. histolytica trophozoites were incubated for 3 h with 0.5% DMSO, 14 μM of tipifarnib and 25 μM of tipifarnib and at the end of incubation trophozoites were allowed to invade the matrigel for 48 h. After 48 h, the migrated cells in the lower chamber were counted and percentage invasion of trophozoites was calculated and plotted (A). Images of trophozoites at 48 h were captured using a Zeiss Axiovert A1 inverted microscope and a Zeiss AxioCam 503 mono digital camera controlled by Zen 2 lite software. Trophozoites were treated for 3 h with 0.5% DMSO (B), 14 μM tipifarnib (C), and 25 μM tipifarnib (D) and migrated trophozoites in lower chambers were imaged at 48 h. Trophozoites were photographed at 10 × magnification. Arrow indicates E. histolytica trophozoites. **P < 0.01 and ***P < 0.001 by Student's t-test compared to 0.5% DMSO-treated E. histolytica trophozoites.