Figure 2.

Functional definition of a novel nonmobile colistin resistance (NMCR‐1) determinant from Shewanella algae. A) PCR assay of nmcr‐1 in S. algae and S. oneidensis. B) Western blot‐based analyses for expression of NMCR‐1 in S. algae. C) SDS‐PAGE (15%) profile of the purified NMCR‐1 protein. D) Comparative analyses of colistin resistance in S. algae and S. oneidensis. E) Expression of nmcr‐1 allows the colistin‐susceptible S. oneidensis to grow on the nonpermissive condition supplemented with up to 8 µg mL⁻¹ of colistin. Bacterial strains were kept on LBA plates with different levels of colistin. A representative result is given from over three independent experiments.