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Comparative analyses for colistin resistance levels in E. coli conferred by an array of different MCR versions. A) Western blot‐aided comparative analyses of functional expression of mcr‐1/2/3/4/5 in vivo. B) Growth viability of E. coli harboring different version of MCR family of enzymes on the LBA plates supplied with varied level of colistin. A representative result is given from three independent experiments. Designation: Vec, pBAD24; WB, Western blot.
