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. 2019 Apr 3;6(11):1900034. doi: 10.1002/advs.201900034

Figure 10.

Figure 10

Functional expression of mcr‐1/2/5 genes is accompanied with bacterial metabolic fitness. Regardless of the presence of the A,E) empty vector pBAD24 or B–D) MCR‐1/2/5, no addition of the inducer arabinose cannot significantly alter bacterial survival in E. coli MG1655. F–H) Confocal microscopy assays illustrate that arabinose (0.2%)‐triggered expression of MCR‐1/2/5 interferes bacterial viability. I) Measurement of the relative ratio of LIVE/DEAD E. coli strains expressing MCR‐1/2/5. 0.2% (w/v) l‐arabinose was added to initiate the expression of mcr‐1/2/5. Bacterial cells were stained with LIVE/DEAD kit, giving the images with confocal laser scanning microscopy. Green and red refer to live and dead cell. Vector refers to pET21. The data were expressed using one‐way analysis of variance (ANOVA) followed by Tukey–Kramer multiple comparisons post hoc test.47 Statistical significance was set at ***p < 0.001.