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. 2019 Apr 6;6(11):1900023. doi: 10.1002/advs.201900023

Figure 4.

Figure 4

miRNA was effectively delivered into CMs by CHO‐PGEA and preform biology function. A) Alignment of mmu‐miR‐182 with putative 3′UTR target sites: 2 sites in FOXO3. miR‐182 seed sequence and the corresponding target sites were indicated in green box. Complementary bases were shown in red color. B) qRT‐PCR shows relative expression of miR‐182 and FOXO3 mRNA in cardiomyocytes after PE stimulation for 48 h. C) Representative FOXO3 expression after overexpressing miR‐182 in heart by western blot (WB). D) qRT‐PCR shows relative expression levels of miR‐182 in H9C2 cells treated with CHO‐PGEA/miR‐182 complexes after 24 h. E) Protein level of FOXO3 in H9C2 cells administered with polycation/miRNA. F) Relative expression of miR‐182 in mouse hearts after intravenous (IV) injection of CHO‐PGEA/miR‐182 complexes (5 nmol) or miR‐182 agomir (5 nmol), (n = 3 per group). *P < 0.05, **P < 0.01, ***P < 0.001 by Student's t‐test or one‐way ANOVO.