Skip to main content
. 2019 Jul;370(1):104–110. doi: 10.1124/jpet.119.256594

Fig. 5.

Fig. 5.

Changes in cAMP activity associated with lipid raft and nonraft plasma membrane domains after β2AR stimulation in cells overexpressing the AKAP-disrupting peptide Ht31. (A) Representative time course of changes in magnitude of FRET response (ΔR/R0) detected by Epac2-MyrPalm and Epac2-CAAX biosensors after activation of β2ARs with 3 nM isoproterenol (Iso) in human ASM cells overexpressing (OE) Ht31 and AC6 OE. Results are normalized to the magnitude of the maximal response produced by forskolin (FSK, 10 µM) and IBMX (100 µM) in the same cell. (B) Size of average FRET responses in control cells (black bars) and cells overexpressing AC6 plus Ht31 (red bars). There were no significant (P > 0.05, Student’s t test with Holm-Sidak correction) differences in the magnitude of the responses to Iso detected by Epac2-MyrPalm in control cells (n/N = 6/6) and cells overexpressing AC6 plus Ht31) (n/N = 10/4) or the magnitude of the responses detected by Epac2-CAAX in control cells (n/N = 7/6) and cells overexpressing Ht31 plus AC6 (n/N = 7/3). Control data from experiments illustrated in Fig. 1.