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. 2019 Jul 15;380(2):115–123. doi: 10.1016/j.yexcr.2019.04.008

Fig. 1.

Fig. 1

Effects of Substance P on the expression of tight junction protein ZO-1 in MRMEC cells. (A, B) Cells were deprived of serum for 24 h and then incubated in the presence of SP or VEGF. MRMEC viability was determined in each group using the MTT assay. Data were normalized by control. (C, D) ZO-1 expression in MRMECs deprived of serum for 24 h, upon treatment with SP (0–40 μM) or VEGF (0–200 ng/ml). (E) ZO-1 expression in MRMECs deprived of serum for 24 h and then incubated in the presence of SP (20 μM), VEGF (100 ng/ml) or SP with VEGF (pre-incubated with SP for 6 h before addition of VEGF). (F, G) p44/p42 MAPK, p38 MAPK and ZO-1 expression in MRMECs treated with SP (20 μM) or VEGF (100 ng/ml). Data represents means ± SD of relative values vs control from 3 independent experiments. *P < 0.05; ***P < 0.001, statistical analysis was performed with one-way ANOVA with Dunn's test for multiple comparisons.