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A
Schematic linear map of mouse mtDNA indicating the relative position of mtRNA transcripts analyzed by northern blot.
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B–E
Northern blot analyses (B and D) and relative quantification (C and E) of mtRNA transcripts in control and Tefm knockout mouse hearts at the age of 4 and 8 weeks (n = 12 mice at each time‐point for each group). 18S rRNA and 5.8S rRNA were used as loading controls, respectively.
Data information: In (C and E), data are presented as mean ± SEM. *
P < 0.05, **
P < 0.01, and ***
P < 0.001; Student's
t‐test. (C) HSP transcripts:
12S rRNA 4 weeks
P = 0.005966044, 8 weeks
P = 0.0806819;
16S rRNA 4 weeks
P = 0.000223004, 8 weeks
P = 0.00243443;
Nd1 4 weeks
P = 0.0352088, 8 weeks
P = 0.000344158;
Cox1 4 weeks
P = 0.000353895, 8 weeks
P = 0.00145773;
Cox2 4 weeks
P = 0.0145193, 8 weeks
P = 0.00578484;
Cox3 4 weeks
P = 0.00834317, 8 weeks
P = 0.000392364;
Nd5/
Cyb 4 weeks
P = 0.0010185, 8 weeks
P = 0.00153049;
Nd5 4 weeks
P = 0.024621, 8 weeks
P = 7.38074E‐05;
Cyb 4 weeks
P = 3.46175E‐06, 8 weeks
P = 0.000991942; LSP transcript:
Nd6 4 weeks
P = 0.000162704, 8 weeks
P = 0.000187855. (E) HSP transcripts:
Tf 4 weeks
P = 0.0297685, 8 weeks
P = 0. 000212544;
Tv 4 weeks
P = 0.842023, 8 weeks
P = 0.269231;
Tl1 4 weeks
P = 0.00921072, 8 weeks
P = 0.000430068;
Tm 4 weeks
P = 0.0084742, 8 weeks
P = 0.000142524;
Tk 4 weeks
P = 0.00297015, 8 weeks
P = 0.00010425;
Ts2 4 weeks
P = 0.0128829, 8 weeks
P = 2.27321E‐05;
Tl2 4 weeks
P = 0.00701324, 8 weeks
P = 0.000411549;
Tt 4 weeks
P = 0.00572118, 8 weeks
P = 0.000807943; LSP transcript:
Tp 4 weeks
P = 0.0427191, 8 weeks
P = 0.0483328;
Te 4 weeks
P = 0.464031, 8 weeks
P = 0.13412;
Ts1 4 weeks
P = 0.0382215, 8 weeks
P = 0.000478237;
Tc 4 weeks
P = 0.00418795, 8 weeks
P = 0.000287525;
Tn 4 weeks
P = 0.00531668, 8 weeks
P = 0.000374367;
Ta 4 weeks
P = 0.00247257, 8 weeks
P = 0.000564597;
Tq 4 weeks
P = 0.00385524, 8 weeks
P = 0.000727981.
