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. 2019 May 13;20(6):e47202. doi: 10.15252/embr.201847202

Figure 2. Overexpression of hTau upregulates phosphorylated STAT1 in vivo .

Figure 2

  • A, B
    AAV‐hTau‐eGFP (AAV‐hTau) or the empty vector AAV‐eGFP (1.13 × 1013 v.g./ml) was stereotaxically injected into hippocampal CA3 of 3‐month‐old C57 mice. After 1 month, the increased levels of STAT1 and pY‐STAT1 in hippocampal total extracts and the nuclear fraction were detected in hTau group by Western blotting (n = 6, Mann–Whitney test).
  • C, D
    The increased STAT1 and pY‐STAT1 in hippocampal total extracts and the nuclear fraction of 12‐month‐old hTau transgenic mice were measured by Western blotting (n = 4, Mann–Whitney test).
  • E, F
    The representative images of STAT1 and pY‐STAT1 in the brain of AD patients probed by co‐immunohistochemical staining and quantitative analysis (hematoxylin stains nuclei, purple; DAB stains the target proteins, brown; n = 5–6 slices). Arrowheads indicated typical nuclear staining of STAT1/pY‐STAT1.
  • G
    The increased AT8 (pS202/pT205), STAT1, and pY‐STAT1 in cortex total extracts of AD patients were measured by Western blotting (n = 3, Student's t‐test).
  • H
    STAT1 mRNA was analyzed by qRT‐PCR in AAV‐hTau‐ or AAV‐eGFP infected hippocampal tissues (n = 6, Mann–Whitney test).
Data information: Data were presented as mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001 vs eGFP, wt, or Ctrl. Source data are available online for this figure.