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. 2019 May 13;20(6):e47202. doi: 10.15252/embr.201847202

Figure 5. Overexpressing hTau increases binding of STAT1 to NMDAR promoters and inhibits the expression of NMDARs.

Figure 5

  • A
    Overexpression of AAV‐hTau increased binding of STAT1 to the promoter regions of GluN1, GluN2A, and GluN2B gene in hippocampal CA3 extracts measured by chromatin immunoprecipitation assay (ChIP) (n = 3 from three independent experiments).
  • B
    Overexpression of hTau or wild‐type STAT1 (WT‐STAT1) inhibits the transcription activity of NMDARs compared with the empty vector control (Ctrl) measured by luciferase activity assay in HEK293 cells (n = 3 from three independent experiments).
  • C–J
    Diagrams show the predicted GAS promoter elements (GASs) for STAT1 in the promoter (−2,000 to +299 bp) of GluN1 (C), GluN2B (E), and GluN2A (G, I). The GASs or the mutant (MUT) plasmids were co‐transfected, respectively, with WT‐STAT1 or its empty vector (Ctrl) into HEK293 cells for 24 h, and then, the luciferase activity was measured (right panels). N = 4 for each group.
Data information: Data were presented as mean ± SD (two‐way analysis of variance (ANOVA) followed by Bonferroni's post hoc test for A‐J). *P < 0.05; **P < 0.01; ***P < 0.001 vs Ctrl; ### P < 0.001 vs wild‐type reporters.