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. 2019 Apr 25;20(6):e47723. doi: 10.15252/embr.201947723

Figure 4. Satellites have varying degrees of effects on regulating centrosomal and cellular abundance of key ciliogenesis factors.

Figure 4

  • A–F
    Effect of satellite loss on centrosomal abundance of proteins in cells serum‐starved for 24 h. Control, IMCD3 KO, and IMCD3 KO stably expressing LAP‐PCM1 cells were fixed and stained with antibodies for the indicated proteins along with anti‐GFP for marking LAP‐PCM1 and anti‐polyglutamylated tubulin or anti‐gamma‐tubulin for marking centrosomes. DNA was stained with DAPI. Scale bar, 1 μm. Both ciliated and unciliated cells were quantified in a blinded manner. Images represent cells from the same coverslip taken with the same camera settings. The centrosomal fluorescence intensity of the indicated proteins was measured in a 3 μm2 square area around the centrosome, and levels are normalized to 1. Results shown are the mean of two independent experiments ± SD (100 cells/experiment, *P < 0.5, **P < 0.01, ***P < 0.001, ns: not significant t‐test). Error bars represent SD. Horizontal line represents mean value of each group. The boxes include data from the 25th to 75th percentiles in each group.
  • G
    Effects of satellite loss on cellular abundance of centrosome proteins. Whole‐cell lysates from control and IMCD3 PCM1 KO cells serum‐starved for 24 h were immunoblotted with the indicated antibodies. Vinculin was used as a loading control. Results shown are the mean of two independent experiments ± SD (*P < 0.5, **P < 0.01, t‐test).