Figure 2. Intestinal epithelial Imp1 deletion attenuates the effects of DSS‐mediated colonic damage.

• A
  Mice were given three cycles of 2.5% dextran sodium sulfate (DSS) in drinking water for 5 days followed by a week of recovery. Mice were analyzed at day 36.
• B
  Imp1 ^ΔIEC mice show significantly less total colitis (7.769 ± 0.4824, n = 13; scored blinded by a pathologist) as compared to Imp1 ^WT mice (9.714 ± 0.8371, n = 7; P‐value = 0.0435).
• C
  Imp1 ^ΔIEC mice show significantly less hyperplasia (1.615 ± 0.1404, n = 13) as compared to Imp1 ^WT mice (2.286 ± 0.2857, n = 7; P‐value = 0.0287).
• D
  Imp1 ^ΔIEC mice show significantly less inflammation score (6.231 ± 0.3608, n = 13) as compared to Imp1 ^WT mice (8 ± 0.6901, n = 7; P‐value = 0.0213).
• E
  Imp1 ^ΔIEC mice show significantly less mononuclear cell infiltration (1.077 ± 0.076, n = 13) as compared to Imp1 ^WT mice (1.714 ± 0.2857, n = 7; P‐value = 0.0128).
• F–J
  qPCR data showing cytokine expression following chronic DSS treatment in colon epithelium of Imp1 ^WT (n = 4) and Imp1 ^ΔIEC mice (n = 6) at day 36 (P‐values are as follows: 0.0109 for Il6, 0.0107 for Il11, and 0.0240 for Il1b).
• K
  Tumors were induced in Imp1 ^WT and Imp1 ^ΔIEC mice using the azoxymethane/dextran sodium sulfate (AOM/DSS) model.
• L
  Tumors were evaluated 14 weeks after the initial AOM injection. Imp1 ^WT (8.235 ± 1.723, n = 17, 5F/12M) and Imp1 ^ΔIEC (7.571 ± 1.337, n = 14, 7F/7M) mice do not show any significant difference in tumor initiation.
• M, N
  Imp1 ^WT and Imp1 ^ΔIEC mice do not show any significant differences in tumor size or load. Imp1 ^WT n = 17, 5F/12M; Imp1 ^ΔIEC n = 12, 7F/7M.

Data information: Data are expressed as mean ± SEM. *P < 0.05, **P < 0.01; by unpaired two‐tailed t‐test.