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. 2019 May 6;20(6):e47074. doi: 10.15252/embr.201847074

Figure EV3. Validation of Atg7 Δ IEC mice.

Figure EV3

  1. Representative western blot comparing Atg7 ΔIEC mice and controls. Atg7 ΔIEC mice show no ATG7 expression and decreased autophagic flux indicated by increased p62 and absence of LC3‐II levels.
  2. Mean fluorescence intensity of CytoID is significantly reduced in epithelial cells From Atg7 ΔIEC mice indicating decreased autophagy (n = 3 per genotype).
  3. Graph showing percentage of DAPI (4′,6‐diamidino‐2‐phenylindole) negative crypt cells (indicating viability) from Imp1 WT (n = 7) and Imp1 ΔIEC mice (n = 8) using flow cytometry with and without 12 Gy radiation. Although 12 Gy radiation significantly decreased viability, there was a no difference in viability between the two genotypes in either condition.
Data information: All data are expressed as mean ± SEM. ****P < 0.0001; by two‐tailed, unpaired t‐test (B) or 1‐way ANOVA with Bonferroni's multiple comparison test (C).