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. 2019 May 29;10:623. doi: 10.3389/fphar.2019.00623

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Copyright © 2019 Ren, McGowan, Li, Zhu, Lu, Zhu, Lin and He

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Alteration of p-STAT3, COX-2, and STAT3 expression post-treatment with SSd. (A) Representative images of immunocytochemical staining of SMMC-7721 cells pre- and post-treatment with AG490, SSd, and IL-6. Control group: Cells treated with PBS; IL-6 group: Cells treated with IL-6 (25 ng/ml); AG90 group: Cells treated with IL-6 (25 ng/ml) + AG490 (25 µmol/L); and SSd group: Cells treated with IL-6 (25 ng/ml) + SSd (5 µg/ml). DAB used as chromogen; original magnification ×200: nuclear immunoreactivity. (B) Results from quantitative analysis of images using ImagePro Plus 7.1 software and data were expressed as arbitrary image units. **P < 0.01, *P < 0.05 compared to the control. Nucleus was stained using hematoxylin (blue), antibody staining (brown).