Figure 2. The effect of ketotifen fumarate on the release of β-hexosaminidase by DENV3-induced RBL-2H3 cells using MOIs ranging from 0.2 × 10⁻² to 1.6 × 10⁻² FFU/cell.

RBL-2H3 cells were pretreated with ketotifen fumarate (300 μM) for 20 min followed by DENV3 infection for 1.5 h to induce degranulation. The percentage of β-hexosaminidase released was calculated against the total amount of β-hexosaminidase. Results are expressed as the mean ± S.E.M. of three independent experiments. ***P<0.005, **P<0.01 and *P<0.05 as compared with their respective DENV3-induced RBL-2H3 groups (black bars).